Part:BBa_K2467000:Design

DsbA DspB emGFP

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal AgeI site found at 316
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 472
  

Design Notes

We needed to incorporate the restriction sites of EcoR1 and BamH1 to allow for incorporation into the pRSETemgfp MCS site.

Source

BBa_K1659211